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哺乳动物细胞含有两种超氧化物歧化酶(SOD),即CuZn—SOD和Mn—SOD,组织细胞SOD活力的高低与其物质代谢的活跃程度密切相关。本实验取3例妊娠后期胎儿的脑、肝、肾、心肌、骨骼肌、肺、脾及胸腺8种组织,用冷生理盐水充分洗去所含血液,采用化学发光法测定各组织的SOD活力,然后组织匀浆在4℃条件下经100 000×g超速离心,取上清直接进行薄层(0.5mm) 聚丙烯酰胺等电聚焦电泳(pH范围为3.5～10.0)。电泳在1400V、35mA、15W、4℃条件下进行,电泳完
Mammalian cells contain two kinds of superoxide dismutase (SOD), namely CuZn-SOD and Mn-SOD. The level of SOD activity in tissue cells is closely related to the activity of substance metabolism. In this experiment, three kinds of tissues of brain, liver, kidney, myocardium, skeletal muscle, lung, spleen and thymus of fetus in the third trimester of pregnancy were washed. The blood was washed thoroughly with cold saline. The chemiluminescence The tissue homogenate was then subjected to ultracentrifugation at 100 000 × g at 4 ° C. The supernatant was directly subjected to isoelectric focusing (0.5 to 5 mm) polyacrylamide isoelectric focusing (pH range: 3.5 to 10.0). Electrophoresis in 1400V, 35mA, 15W, 4 ℃ conditions, electrophoresis finished