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借助吸附测定、动电测定和浮选试验研究了T t 菌与闪锌矿和方铅矿的作用。试验表明 ,吸附在方铅矿和闪锌矿表面上的T t 菌的数量与 pH无关。但是 ,细菌细胞在方铅矿上的吸附量比在闪锌矿上的吸附量高 1个数量级 ,T t 菌在方铅矿和闪锌矿上的吸附等温线表现为兰格缪尔特性。电泳测定表明 ,方铅矿和闪锌矿的等电点位于pH 2附近 ,T t 菌的等电点位于pH3附近。与T t 菌作用后 ,矿物的等电点向高pH偏移 ,这表明细菌在矿物表面上特效吸附。电泳迁移率测定还查明 ,随细菌细胞作用时间的增长 ,电泳迁移率负值减小。在与矿物作用不同时间的细胞的电泳迁移率也具有类似的变化规律。与闪锌矿作用后的细胞的电泳迁移率比与方铅矿作用后的细胞电泳迁移率负值要小些。在与T t 菌作用 1h不影响闪锌矿的浮选回收率 ,而与T t 菌作用 1h后在pH 5～ 11范围内 ,方铅矿的浮选几乎完全被抑制。方铅矿和闪锌矿人工混合样浮选分离试验表明 ,在T t 菌存在时可以从方铅矿中优先浮选闪锌矿。
The effect of Tt bacteria on sphalerite and galena was investigated by means of sorption measurements, electrokinetic measurements and flotation experiments. Experiments have shown that the amount of Tt bacteria adsorbed on the surfaces of galena and sphalerite has nothing to do with pH. However, bacterial cells adsorbed on galena one order of magnitude higher than those adsorbed on sphalerite, and the adsorption isotherm of T t bacteria on galena and sphalerite was characterized by the Langmuir feature. Electrophoresis showed that the isoelectric point of galena and sphalerite was near pH 2 and the isoelectric point of T t was located near pH 3. After interaction with Tt bacteria, the isoelectric point of the mineral shifts to a high pH, indicating that the bacteria adsorb specifically on the mineral surface. Electrophoretic mobility measurement also found that, with the increase of bacterial cell action time, negative electrophoretic mobility decreased. The electrophoretic mobility of cells at different times of mineralization also had similar changes. The electrophoretic mobility of cells with sphalerite was smaller than the negative values of cell electrophoretic mobility after galena. The flotation recovery of sphalerite was not affected by 1 h incubation with Tt, but the flotation of galena was almost completely inhibited at pH 5-11 after 1 h incubation with Tt. Artificial mixed flotation separation of galena and sphalerite shows that sphalerite can be preferentially flotated from galena in the presence of Tt bacteria.