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目的 :深入探讨胸腺在重症肌无力 (MG)发病中的作用。方法 :采用SDS PAGE电泳对MG患者胸腺提取液进行分析 ,并用MTT法研究了胸腺提取液对外周血单个核细胞和胸腺细胞增殖的影响。结果 :30 %MG患者胸腺提取液中存在异常蛋白 ,其相对分子质量小于 144 0 0 ,而在先天性心脏病患者胸腺细胞培养上清中未见这种蛋白。异常蛋白组MG患者的胸腺提取液对正常人外周血单个核细胞 (PBMC)增殖有显著的抑制作用 ,与非自身免疫病患者相比 ,差异有显著性 (P <0 .0 1) ,与其他非异常蛋白组MG患者相比差异有显著性 (P <0 .0 5 ) ,但对胸腺细胞的增殖未见显著差异。结论 :MG患者免疫功能的异常与胸腺提取液中小分子异常蛋白的存在有关 ,与MG的发病有关。
Objective: To investigate in depth the role of thymus in the pathogenesis of myasthenia gravis (MG). Methods: The thymus extract of MG patients was analyzed by SDS PAGE electrophoresis, and the effect of thymus extract on the proliferation of peripheral blood mononuclear cells and thymocytes was studied by MTT assay. Results: There was abnormal protein in thymus extract of 30% MG patients, the relative molecular mass was less than 14400, but not in thymocyte culture supernatant of patients with congenital heart disease. Thymus extract from MG patients with abnormal proteome significantly inhibited the proliferation of peripheral blood mononuclear cells (PBMCs) in normal people, which was significantly different from those in non-autoimmune diseases (P <0.01) The other non-abnormal protein MG patients compared with the difference was significant (P <0. 05), but there was no significant difference in the proliferation of thymocytes. Conclusion: The abnormal immune function in patients with MG is related to the presence of small molecule abnormal protein in thymus extract, which is related to the pathogenesis of MG.