目的　从外周血单核细胞中克隆人IL 1 8成熟编码序列cDNA ,对其进行测序并构建原核表达载体 ,进行原核表达。方法　从人外周血单核细胞中分离总RNA ,进行RT PCR获得人IL 1 8cDNA。测序证实其结果正确后 ,构建原核表达载体。E .coli的表达产物通过SDS PAGE、WesternBlot证实。结果　所克隆的人IL 1 8cDNA编码序列经测序证实与GenBank所报道的序列完全一致 ,细菌表达产物经SDS PAGE证实其大小约为 1 8.3KDa,WesternBlot进一步证实了所表达产物的正确性。结论　本研究结果为进一步探讨IL 1 8的生物学活性和特性奠定了基础 ,同时亦为利用人IL 1 8进行免疫基因治疗的研究打下了良好的基础。 OBJECTIVE: To clone human IL-18 mature cDNA from peripheral blood mononuclear cells (PBMCs), to sequence and construct a prokaryotic expression vector for prokaryotic expression. Methods Total RNA was isolated from human peripheral blood mononuclear cells and subjected to RT PCR to obtain human IL18 cDNA. Sequencing confirmed its correct results, the construction of prokaryotic expression vector. The E. coli expression product was confirmed by SDS PAGE and Western Blotting. Results The sequence of human IL 18 cDNA cloned was confirmed by sequencing. Genomic DNA was confirmed by SDS PAGE. The size of the expressed product was about 8.3KDa. Western Blot further confirmed the correctness of the expressed product. Conclusion The results of this study lay a foundation for further exploration of the biological activity and characteristics of IL-18, and laid a good foundation for the research of immunotherapy with human IL-18.