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通过增加外源CaCl_2和钙离子螯合剂EGTA处理后,研究香蕉幼苗在60 mmol/L NaCl人工模拟的盐胁迫0、4 h、8 h、12 h、24 h和48 h不同时间下,测定其叶片和根的CaM和Ca~(2+)-ATPase基因的相对表达量。结果表明,在NaCl胁迫过程中,香蕉幼苗在正常的生长条件下,其根和叶片的CaM基因和Ca~(2+)-ATPase基因均有表达。巴西蕉幼苗根在盐胁迫过程中Ca M基因没有发生显著变化,但是粉蕉根却发生了显著变化,这可能与粉蕉耐盐性高于巴西有关。随着盐胁迫时间的延长,巴西蕉和粉蕉幼苗根的Ca~(2+)-ATPase基因的相对表达量均呈现先上升后下降的趋势。
By adding exogenous CaCl 2 and calcium ion chelator EGTA, the banana seedlings were treated with 60 mmol / L NaCl artificial salt stress for 0, 4, 8, 12, 24 and 48 h Relative Expression of CaM and Ca ~ (2 +) - ATPase Genes in Leaves and Roots. The results showed that both the CaM gene and the Ca ~ (2 +) - ATPase gene were expressed in roots and leaves of banana seedlings under normal conditions during the NaCl stress. There was no significant change of Ca M gene in the seedling roots of Brazil banana under salt stress, but there was a significant change in the banana roots, which may be related to the higher salt tolerance of banana plantain than that in Brazil. With the prolongation of salt stress, the relative expression of Ca ~ (2 +) - ATPase gene in seedling roots of Banana and Banana showed the trend of first increasing and then decreasing.