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为观察灵芝多糖 (GL B7)体外对小鼠腹腔巨噬细胞 (MΦ)蛋白激酶 A(PKA)活性的影响 ,采用反相离子对高效液相色谱法测定 MΦ中 RKA活力。结果表明 GL B7(40 mg/ L )能引起小鼠腹腔 MΦ中 PKA活性明显升高 ,10min达峰值 ,30 min恢复到基础水平。提示灵芝多糖的免疫增强作用与其增强 MΦ中 PKA活性有关。
In order to observe the effect of Ganoderma lucidum polysaccharide (GL B7) on the activity of mouse peritoneal macrophages (MΦ) protein kinase A (PKA) in vitro, reversed phase ion pair high performance liquid chromatography was used to determine the RKA activity in MΦ. The results showed that GL B7 (40 mg/L) could induce a significant increase in PKA activity in MΦ of the peritoneal cavity, peaked at 10 min, and returned to basal level at 30 min. It suggests that the immunoenhancing effect of Ganoderma lucidum polysaccharides is related to its enhancement of PKA activity in MΦ.