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目的:系统地观察研究脂多糖注射法制作温病实验动物模型的方法。方法:观察实验兔接受单次、两次和多次脂多糖静脉注射后的体温与其它症状、体征的变化,分析动物病情特点及其与温病证型之间的联系,对部分实验动物进行炎症细胞因子水平检测和肺脏、肝脏组织的病理检查。结果:绝大部分实验兔出现了伴随不同症状、体征特点的发热,符合温病实验动物制作的要求。结论:实验兔单次注射后类似温病卫气分证候,两次注射后类似营血分证候,多次注射后类似留恋气分或肝肾阴伤证,大剂量注射后类似于内闭外脱证。
OBJECTIVE: To systematically observe and study the method of producing lipopolysaccharide injection animal model of warm sickness. Methods: The changes of body temperature and other symptoms and signs of experimental rabbits after single, double and multiple intravenous injection of lipopolysaccharide were observed. The characteristics of animal disease and its relationship with the syndrome of warm-disease were analyzed. Some experimental animals Inflammatory cytokines levels and lung, liver tissue pathological examination. Results: The majority of experimental rabbits had fever with signs and symptoms, which were in line with the requirements of warm animal experiment animals. Conclusion: After a single injection, experimental rabbits are similar to Wen Wei syndrome with warm syndrome, similar to Yingbian syndrome after two injections, similar to those with nausea or liver and kidney after multiple injections, Close outside the card.