本实验使用特异性非常高的抗β-内啡肽抗血清,用放射免疫法测定人体液和组织中的β-内啡肽含量。从死于非中枢神经系统疾病的4例男性和3例女性尸体取脑和垂体组织,用显微解剖取得的下丘脑各个部分,以及其它脑区都分别加入0.5ml 0.1N过氯酸制成匀浆,用微声器给予声波5～10秒。制匀浆时垂体前叶用10ml 0.1N过氯酸,垂体后叶用0.5～2.5ml。于5℃, This experiment used a very specific anti-β-endorphin antiserum, radioimmunoassay determination of human body fluid and tissue β-endorphin content. Brain and pituitary tissues were obtained from 4 males and 3 females dead from diseases of the non central nervous system. Each part of the hypothalamus obtained by microdissection, and other brain regions, were each supplemented with 0.5 ml of 0.1 N perchloric acid Homogenized, with a micro-sound waves to give 5 to 10 seconds. Preparation of homogenate anterior pituitary with 0.1ml of 0.1N perchloric acid, pituitary gland with 0.5 ~ 2.5ml. At 5 ° C,