论文部分内容阅读
目的：：建立归芍地黄丸中山茱萸的定性和定量测定方法。方法：采用TLC对归芍地黄丸中山茱萸进行定性鉴别,并采用HPLC测定山茱萸中莫诺苷、马钱苷的含量。结果： TLC色谱斑点清晰,分离度好,专属性强；莫诺苷对照品在0.010~2.620μg范围内线性关系良好(r =0.9999),平均回收率为102.75%,RSD 为1.40%(n =6)。马钱苷对照品在0.009~2.170μg范围内线性关系良好(r=0.9999),平均回收率为103.59%,RSD为1.10%(n=6)。结论：本方法准确、简单、可行,重复性好,可有效控制该制剂中山茱萸的质量。“,”Objective:To establish the qualitative and quantitative methods for Corni Fructus in Guishao Dihuang pills. Methods:A TLC method was used to identify morroniside and loganin. An HPLC method was used to determine the content of morroniside and lo-ganin in Corni Fructus. Results:The TLC spots were clear with good separation and specificity. The linear range of morroniside was within 0. 010-2. 620 μg (r=0. 999 9) and the average recovery was 102. 75% (RSD=1. 40%, n=6). The linear range of loganin was within 0.009-2.170 μg (r =0.999 9), and the average recovery was 103.59% (RSD =1.10%, n =6). Conclusion: The method is accurate, simple and feasible with good repeatability, which can be used for the quality control of Corni Fructus in the prepa-ration.