论文部分内容阅读
本研究在培养分泌抗甲状腺球蛋白单克隆抗体（ Ｉｇ Ｇ 型） 杂交瘤 Ｈ４ 株时， 加入不同浓度（０ ％ 、２ ．５ ％ 、５ ％ 、１０ ％ 、２０ ％ ） 的人胎盘培养上清液（ Ｈ Ｐ Ｓ） ，培养不同的时间（２４ｈ 、４８ｈ 、７２ｈ 、９６ｈ）后取上清液，用 Ｅ Ｌ Ｉ Ｓ Ａ 法检测各培养上清液中抗体含量。结果表明， 低浓度（２ ．５ ％ 、５ ％ ） Ｈ Ｐ Ｓ 能够使 Ｈ４ 单抗分泌增强（ Ｐ＜ ０ ．０１） ；而高浓度（２０ ％ ） 的 Ｈ Ｐ Ｓ 能够使 Ｈ４ 单抗分泌减弱（ Ｐ＜ ０ ．０１） 。研究表明， Ｈ Ｐ Ｓ 在体外可作用于有亲代 Ｂ 细胞特性的杂交瘤细胞株 Ｈ４ ，对 Ｈ４ 株分泌单抗的量可由于 Ｈ Ｐ Ｓ 浓度不同而有促进或抑制作用， 以此探讨 Ｈ Ｐ Ｓ 对 Ｂ 细胞分泌抗体的影响， 以期阐明胎盘在保护胎儿中的作用机制。
In this study, we cultured human H4 strain secreting anti-thyroglobulin monoclonal antibody (Ig G) by adding human placental culture supernatant of different concentrations (0%, 2.5%, 5%, 10% and 20% (H P S). The supernatants were harvested at different time (24h, 48h, 72h, 96h), and the antibody content in each culture supernatant was detected by ELISA. The results showed that low concentration of H P S (2.5%, 5%) enhanced the secretion of H4 monoclonal antibody (P <0.01), whereas high concentration (20%) of H P S reduced the secretion of H4 monoclonal antibody (P <0 .01). Studies have shown that, H P S in vitro can be used to have the characteristics of the parental B cell hybridoma cell line H4, H4 monoclonal antibody secretion due to the concentration of H P S have a role in promoting or inhibiting to explore H P S on the secretion of antibodies to B cells in order to clarify the role of the placenta in the protection of the fetus mechanism.