论文部分内容阅读
目的探讨大鼠闭合性颅脑损伤后脑组织微管相关蛋白-2(MAP-2)表达变化规律。方法建立大鼠闭合性颅脑挫伤模型6,4只Wistar大鼠随机分为损伤后0.5h、6h1、2h、1d3、d7、d1、4d7个损伤组及对照组,每组8只。应用免疫组化和蛋白印记Western blot法及图像分析技术,检测脑挫伤后各设定时间点挫伤脑组织中MAP-2的变化。结果免疫组化染色显示:对照组大鼠脑组织中可见MAP-2阳性表达;实验组伤后0.5h,挫伤灶及周围MAP-2阳性表达下降,伤后6h阳性表达降至最低,伤后12h,阳性表达逐渐增多,伤后14d,仍保持较高水平;蛋白印记结果与免疫组化结果一致。统计学分析显示:MAP-2的表达各损伤组与对照组比较,差异均有统计学意义(P<0.05),伤后12h、3d及14d与上一组比较,差异有统计学意义(P<0.05)。结论大鼠脑挫伤后14d内,MAP-2在脑组织挫伤灶及其周围呈现先减少后逐渐增多的表达变化,可作为推断脑挫伤经过时间的参考指标之一。
Objective To investigate the changes of the expression of MAP-2 in rat brain after closed head injury. Methods To establish a rat model of closed cranial contusion. Sixty-four Wistar rats were randomly divided into seven groups of injury (0.5h, 6h1, 2h, 1d3, d7, d1 and 4d) and control group (n = 8). Immunohistochemistry and Western blot were used to detect the changes of MAP-2 in brain tissue after contusion. Results Immunohistochemical staining showed that the expression of MAP-2 in the brain tissue of the control group was positive. The expression of MAP-2 in the contusion lesion and the surrounding MAP-2 in the experimental group decreased 0.5h after injury, the expression of MAP-2 decreased to the lowest at 6h after injury, 12h, the positive expression gradually increased, 14d after injury, remained at a high level; Western blot results consistent with the results of immunohistochemistry. Statistical analysis showed that the expression of MAP-2 in each injury group compared with the control group, the difference was statistically significant (P <0.05), 12h, 3d and 14d after injury compared with the previous group, the difference was statistically significant (P <0.05). Conclusion Within 14 days after traumatic brain injury, MAP-2 expression decreases first and then gradually increases in the contusion of the brain tissue, which may be used as a reference index to infer the elapsed time of cerebral contusion.