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奈瑟菌表面蛋白A(NeisseriaSurfaceProteinA,NspA)是新近发现的一个具有较强免疫原性和保守性的抗原,是淋球菌疫苗研究的理想侯选者之一[1]。现克隆淋球菌NspA全长基因,为进一步的淋球菌基因疫苗研究奠定基础。一、材料与方法1.质粒与细胞株:真核细胞表达质粒pCMVscript购自Str
Neisseria Surface Protein A (NspA) is a recently discovered antigen with strong immunogenicity and conservation. It is one of the ideal candidates for gonococcal vaccine research [1]. Now Neisseria gonorrhoeae NspA full-length gene, to lay the foundation for further gonococcal genetic vaccine research. I. Materials and Methods 1. Plasmids and Cell Lines: Eukaryotic Expression Plasmid pCMVscript was purchased from Str