NMR在生物学中的应用是从70年代发展起来的。~(31)P-NMR法因其共振谱线简单明了,灵敏度高,能无损伤地监控生物活组织内的与能量代谢密切相关的含磷化合物浓度的动态变化,而成为在生理条件下测定组织内部动力学参量的重要方法。本文通过介绍离体灌流标本和在体组织器官的~(31)P-NMR的具体应用实例,集中反映了在这一领域研究的概况和新近的研究成果。 The application of NMR in biology has developed from the 1970s. The ~ (31) P-NMR method is simple and sensitive because of its resonance spectrum, which can monitor the dynamic changes of concentration of phosphorus-containing compounds in the living tissue closely related to energy metabolism without any damage, An important method of organizing internal kinetic parameters. In this paper, by introducing the specific application examples of ex vivo perfusion specimens and ~ (31) P-NMR in the tissues and organs of the body, a general survey and recent research results in this area are summarized.