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目的了解2013年江西省HIV-1耐药株的传播水平。方法采用WHO的二项顺序抽样法,随机抽取2013年江西省16岁~25岁符合耐药警戒线调查标准的HIV-1感染者血浆样本,提取病毒RNA,采用RT-PCR和巢式PCR扩增HIV pol区基因片段进行原发耐药基因变异分析。结果 47个研究对象的HIV亚型有CRF07_BC、CRF01_AE和B’亚型,分别占51.06%(24/47)、42.55%(20/47)和6.38%(3/47),其中CRF07_BC和CRF01_AE为主要亚型;不同传播途径的感染者中,HIV亚型分布差异无统计学意义。47份样本中仅有1份来自异性性接触感染者的样本存在RT区耐药突变位点(M184V)。结论 2013年江西省HIV-1耐药株的传播水平为低度(<5%),个体在抗病毒治疗前尚不需要进行耐药检测。但是随着抗病毒治疗规模的逐步扩大,有必要继续加强HIV耐药株传播的监测工作。
Objective To understand the transmission of HIV-1 resistant strains in Jiangxi Province in 2013. Methods According to the bi-sequence sampling method of WHO, plasma samples of HIV-1 infected persons from 16 years old to 25 years old in Jiangxi Province in accordance with the investigation of drug resistance alert line were randomly drawn from 2013 to 2008, and viral RNA was extracted. RT-PCR and nested PCR Increased HIV pol region gene fragments of primary resistance gene mutation analysis. Results The HIV subtypes of 47 subjects were CRF07_BC, CRF01_AE and B ’subtypes, accounting for 51.06% (24/47), 42.55% (20/47) and 6.38% (3/47) respectively, of which CRF07_BC and CRF01_AE were Main subtypes; HIV infection in different routes of transmission, HIV subtype distribution was not statistically significant. Only 1 out of 47 samples had RT-resistant mutations (M184V) in samples from heterosexual contacts. Conclusions The level of HIV-1 drug-resistant strains in Jiangxi Province was low (<5%) in 2013, and there was no need for drug resistance testing before antiviral treatment. However, with the gradual expansion of antiviral treatment, it is necessary to continue monitoring the spread of HIV-resistant strains.