论文部分内容阅读
本文探讨了在常规超薄切片时醋酸双氧铀块染对肝糖原保存的影响。 实验方法是取小白鼠肝脏,用2.5％戊二醛二甲砷酸钠缓冲液固定1小时,0.1M二甲砷酸钠缓冲液冲洗2小时,Millonig1％锇酸液后固定1小时,水洗15分钟后用不同溶液的醋酸双氧铀的酒精溶液,水溶液和Michaelise的醋酸巴比妥钠缓冲液,在4℃下进行不同时间的块染色,经各度酒精脱水,氧化丙烯置换,Epon812包埋,切片后用40％酒精醋酸双氧铀饱和液及Reynolds柠檬铅双重染色。
This article discusses the effect of uranyl acetate block on the preservation of hepatic glycogen in conventional ultrathin sections. The experimental method is to take the liver of rat, fix it with 2.5% glutaraldehyde sodium dimethylarsinate buffer for 1 hour, rinse with 0.1M sodium cacodylate buffer for 2 hours, fix it with Millonig1% citric acid for 1 hour, and wash with water. Minutes later, different solutions of uranyl acetate in alcohol solution, aqueous solution and Michaelise’s barbital acetate buffer were used to stain at 4°C for different periods of time. After each degree of alcohol dehydration, propylene oxide was replaced and Epon812 was embedded. After staining, they were stained with 40% alcohol saturated uranyl acetate and Reynolds lemon lead.