近年来,体外受精(IVF)技术在促排卵、受精和胚胎培养等方面取得了很大进展,临床妊娠率也得到了提高,但每个移植周期的妊娠率仍不理想,其影响因素值得进一步研究。卵母细胞的质量是影响成功率的重要因素之一。它受体内激素水平的精密调节,采集到的卵子是否成熟,直接影响到胚胎质量。目前,常规IVF一般是在取卵后4~6 h进行。但近年来有学者认为卵子体外培养2~3 h受精~([1]),甚或培养12 h受精对实验室指标与 In recent years, in vitro fertilization (IVF) technology has made great progress in ovulation induction, fertilization and embryo culture, clinical pregnancy rate has also been improved, but the pregnancy rate is still not ideal for each transplant cycle, the impact of factors worth further the study. The quality of oocytes is one of the important factors affecting the success rate. It is closely regulated by hormone levels in the body, whether the collected eggs are mature and directly affect the quality of embryos. At present, routine IVF is generally carried out 4 to 6 h after ovulation. However, in recent years, some scholars believe that in vitro fertilization eggs 2 ~ 3 h ~ ([1]), or even cultured 12 h fertilization of laboratory indicators and