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目的研究急性肠系膜上动脉夹闭后全身应用碱性成纤维细胞生长因子(bFGF)对肠道和肝的保护作用.方法采用夹闭大鼠肠系膜上动脉40 min 与再灌注72 h 动物模型.72只大鼠随机分成 bFGF 治疗组,肝素生理盐水对照治疗组以及假手术组.用肝功能指标,血浆 TNFa 含量,组织细菌学定量以及病理学方法评价伤后不同时间的治疗效果.结果经 bFGF 治疗的大鼠伤6 h 与24 h 的肝功能损伤较对照明显减轻,血浆 TNFa 含量显著减少.脏器细菌培养以现经bFGF 治疗的大鼠,其肠道细菌迁移至肝脾与淋巴结的发生率较对照治疗组明显下降.结论 bFGF 能显著减轻肠道缺血再灌注对肠道和肝形态与功能损伤.其作用机制可能与 bFGF 的促分裂效应与非促分裂激素样活性有关.
Objective To study the protective effect of bFGF on the intestine and liver after clipping the superior mesenteric artery in rats.Methods The rat model was established by clipping the superior mesenteric artery of rats for 40 min and reperfusion for 72 h.72 The rats were randomly divided into bFGF treatment group, heparin saline control treatment group and sham operation group.The liver function, plasma TNFa level, histological bacteriological and pathological methods were used to evaluate the therapeutic effect at different time points after bFGF treatment Of the rats were injured 6 h and 24 h after liver injury was significantly reduced compared with the control, plasma TNFa levels were significantly reduced organ culture to bFGF-treated rats, the intestinal bacteria migrate to the incidence of liver and spleen and lymph nodes Compared with the control group decreased significantly.Conclusion bFGF can significantly reduce intestinal ischemia-reperfusion injury of the morphology and function of the intestine and liver.The mechanism may be associated with the mitogenic effect of bFGF and non-mitogenic hormone-like activity.