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目的:研究miR-214对鼻咽癌细胞CNE2凋亡的影响及机制。方法:RT-PCR检测鼻咽癌细胞CNE2和正常鼻咽上皮细胞NP69细胞中miR-214的表达;通过miR-214inhibitor转染鼻咽癌细胞CNE2,流式细胞仪(FCM)检测鼻咽癌细胞凋亡变化;并用Western印迹法检测细胞中Bcl-2和Bax蛋白的表达变化。结果:正常鼻咽上皮细胞明低表达miR-214,而鼻咽癌CNE2细胞中明显高表达miR-214(P<0.05),miR-214inhibitor可浓度依赖性的抑制miR-214的表达(P<0.05)。40μmol/L和5μmol/L的miR-214inhibitor组细胞凋亡率分别为:(45.3±9.1)%和(12.3±3.8)%,而NC inhibitor组细胞的凋亡率为(5.1±0.4)%。miR-214inhibitor可下调Bcl-2的表达(P<0.05),对Bax的表达无明显影响。结论:miR-214可能通过下调Bcl-2的表达从而诱导CNE2细胞凋亡,这可能为鼻咽癌的治疗提供新靶点。
Objective: To investigate the effect of miR-214 on apoptosis of nasopharyngeal carcinoma cell line CNE2 and its mechanism. Methods: The expression of miR-214 in nasopharyngeal carcinoma cell line CNE2 and normal nasopharyngeal epithelial cell line NP69 was detected by RT-PCR. The nasopharyngeal carcinoma cell line CNE2 was transfected by miR-214inhibitor and the expression of miR-214 was detected by flow cytometry (FCM) The changes of Bcl-2 and Bax protein expression were detected by Western blot. Results: The normal nasopharyngeal epithelial cells showed low expression of miR-214, while miR-214 was highly expressed in CNE2 cells (P <0.05). MiR-214inhibitor inhibited the expression of miR-214 in a concentration-dependent manner (P < 0.05). The apoptotic rate of miR-214inhibitor group was (45.3 ± 9.1)% and (12.3 ± 3.8)%, respectively, while that of NC inhibitor group was (5.1 ± 0.4)%. miR-214inhibitor down-regulated the expression of Bcl-2 (P <0.05), but had no effect on the expression of Bax. Conclusion: miR-214 may induce CNE2 cell apoptosis by down-regulating the expression of Bcl-2, which may provide a new target for the treatment of nasopharyngeal carcinoma.