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High peak-to-average power ratio (PAPR) is the main disadvantage in orthogonal frequency-division multiplexing (OFDM) communication systems, which also exists in OFDM-radio over fiber (RoF) systems. In this paper, we firstly analyze the impact of high PAPR on a 40 GHz OFDM-RoF system, and then describe the theory of Nyquist pulse shaping technology for reducing PAPR. To suppress PAPR further, an improved Nyquist pulse shaping technology is proposed, in which the distribution of original-data amplitude is changed by properly selecting the time-limited waveforms of the different subcarriers. We firstly apply the improved Nyquist pulse shaping technology to an OFDM-RoF system. The simulation results show that PAPR is effectively reduced by more than 2 dB with the bit error rate (BER) declining by about 0.125%.