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目的探究绿茶对微囊藻毒素 LR(MC-LR)诱导肝肾氧化损伤的拮抗效应。方法 40只雄性小鼠分为空白对照组、MC-LR 染毒组(MC-LR)、绿茶低剂量拮抗组(MC-LR+绿茶2)、绿茶高剂量拮抗组(MC-LR+绿茶12)共4组,实验第1天起 MC-LR+绿茶2、MC-LR+绿茶12组即每日给予2 g/L 和12 g/L 的绿茶自由饮用,连续18 d。自第6天开始,MC-LR、MC-LR+绿茶2、MC-LR+绿茶12组均每日给予 MC-LR 10 μg/kg 腹腔注射1次,空白对照组给予二甲砜腹腔注射,连续13 d。第19天眶静脉取血后处死动物,对脏器系数、血清生化指标、抗氧化酶及脂质过氧化物水平、肝肾组织病理改变等指标进行检测和分析。结果绿茶明显拮抗 MC-LR 所致小鼠体重的下降。绿茶明显拮抗 MC-LR 造成的血清丙氨酸转氨酶、天冬氨酸转氨酶和肌酐的升高。绿茶可抑制 MC-LR 致小鼠脂质过氧化物丙二醛水平的升高,较 MC-LR 染毒组 MDA 均值 13.98 nmol/ml 相比差异有统计学意义。绿茶高剂量拮抗明显升高血清谷胱甘肽及超氧化物歧化酶水平,其均值分别为467.29 mg/L 和139.22 U/ml,与空白、MC-LR 染毒组相比差异有统计学意义。给予绿茶可使 MC-LR 所致的肝肾病理损伤减轻。结论绿茶可提高抗氧化酶活性,清除体内自由基,减轻氧化损伤,从而明显拮抗 MC-LR诱导的肝肾毒性损伤。
Objective To explore the antagonism effect of green tea on liver-kidney oxidative injury induced by microcystins LR(MC-LR). Methods Forty male mice were divided into blank control group, MC-LR exposure group (MC-LR), green tea low-dose antagonist group (MC-LR+green tea 2), green tea high-dose antagonist group (MC-LR+green tea 12) in total In group 4, from the first day of experiment, MC-LR+ green tea 2 and MC-LR+ green tea 12 groups were given 2 g/L green tea and 12 g/L green tea daily for 18 days. From the 6th day, MC-LR, MC-LR+ green tea 2 and MC-LR+ green tea 12 groups were given MC-LR 10 μg/kg intraperitoneally once a day, and blank control group was given intraperitoneal injection of dimethyl sulfone, which was continuous 13 times. d. On the 19th day, the animals were sacrificed after taking blood from the iliac veins. The indexes of organs, serum biochemical indexes, levels of antioxidant enzymes and lipid peroxides, pathological changes of liver and kidney tissues were detected and analyzed. Results Green tea significantly antagonized the decrease in body weight induced by MC-LR in mice. Green tea significantly antagonized the elevation of serum alanine aminotransferase, aspartate aminotransferase, and creatinine caused by MC-LR. Green tea can inhibit the increase of lipid peroxidation of malondialdehyde in mice induced by MC-LR. Compared with the MC-LR exposure group, the average MDA value of 13.98 nmol/ml was statistically significant. The high-dose antagonism of green tea significantly increased the levels of serum glutathione and superoxide dismutase, and the mean values were 467.29 mg/L and 139.22 U/ml, respectively, which were significantly different from the blank and MC-LR exposure groups. . Green tea can reduce the pathological damage of liver and kidney caused by MC-LR. Conclusion Green tea can increase the activity of antioxidant enzymes, remove free radicals from the body, reduce oxidative damage, and antagonize the hepatorenal and nephrotoxic damage induced by MC-LR.