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本文报道应用放射性核素铈(~(141)Ce)标记硝酸铈[~(141)Ce(NO_2)_2),利用ICP-AES法及植物生理生化分析技术,研究测定了稀土元素(REE)在甘蔗体内各器官的吸收、分配、残留累积和REE处理对甘蔗叶片细胞膜透性、伤流量,叶片自由水和束缚水含量以及游离脯氨酸含量等抗性生理指标的影响。甘蔗叶面喷施REE铈后的第1～7天,根、茎、叶器官吸收累积铈量是随着时间的增加而增加,第7天达吸收最大值,随后开始缓慢下降。甘蔗植株对REE铈的吸收率仅为0.836％～2.165％。REE铈在甘蔗叶片有较多的分配,其次为茎,最小为根部;REE在甘蔗叶片有较多的残留累积量,茎(甘蔗渣,下同)次之,根部又次之,蔗糖汁中最小,100～900ppm浓度范围的REE处理,甘蔗叶、茎、根及蔗糖汁中的REE残留累积量是随着REE处理浓度的增加而增加。当处理浓度在900ppm以上时(900～1 500ppm),REE在蔗糖汁中的残留量趋于平衡饱和状态。一定浓度的REE处理甘蔗可显著地提高甘蔗伤流量,降低甘蔗叶片细胞膜透性,减少叶片中自由水含量,提高束缚水和叶片游离脯氨酸含量,从而提高甘蔗的抗逆性能和单位面积产量。
In this paper, we report the determination of the content of rare earth elements (REE) by using the method of ICP-AES and physiological and biochemical analysis of cerium nitrate (~ (141) Ce (NO2) 2) Effects of Absorption, Distribution, Residue Accumulation and REE of Organs in Sugarcane on Resistant Physiological Indexes of Cell Membrane Permeability, Injury, Leaf Free Water and Bound Water Content and Free Proline Content in Sugarcane Leaves. From the first day to the seventh day after the application of REE cerium on the leaves of sugarcane, the amount of accumulated and absorbed cerium in roots, stems and leaves increased with the increase of time, reaching the maximum value on the seventh day and then began to decline slowly. The absorptivity of sugarcane plant to REE cerium is only 0.836% ~ 2.165%. REE cerium has more distribution of sugarcane leaves, followed by the stem, the smallest for the root; REE in sugarcane leaves have more residual accumulation, stem (bagasse, the same below) followed by root followed by sucrose juice The REE concentration in the range of 100-900ppm was the lowest. The accumulation of REE in sugarcane leaf, stem, root and sucrose juice increased with the increase of REE concentration. When the treatment concentration is above 900ppm (900 ~ 1500ppm), the residual amount of REE in sucrose juice tends to balance and saturate. The treatment of sugarcane with certain concentration of REE significantly increased the trapping rate of sugarcane, reduced the membrane permeability of sugarcane leaves, decreased the content of free water in leaves and increased the content of free proline in irreducible water and leaves, thus improving the stress resistance of sugarcane and yield per unit area .