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为探明虫螨腈对甜菜夜蛾幼虫体内生化代谢的影响及酶抑制剂对虫螨腈的增效潜力,采用点滴法测定了虫螨腈对甜菜夜蛾3、4、5龄幼虫的毒力和顺丁烯二酸二乙酯(DEM)、增效醚(PBO)、磷酸三苯酯(TPP)对虫螨腈的增效作用,并采用生化分析法测定了LD25和LD50剂量虫螨腈对甜菜夜蛾体内保护酶系和解毒酶系的影响。结果表明,虫螨腈对甜菜夜蛾不同龄期幼虫的毒力差异不大,处理后24h和48h的LD50基本相同。PBO对虫螨腈有拮抗作用,增效比为0.21,而DEM和TPP对虫螨腈无增效作用。虫螨腈对甜菜夜蛾幼虫体内超氧化物歧化酶具有激活作用,对过氧化氢酶、过氧化物歧化酶及羧酸酯酶具有先抑制后诱导的作用;虫螨腈处理后6～24h,谷胱甘肽-S-转移酶活性显著提高,最高为对照的1.85倍;处理6h后,甜菜夜蛾体内细胞色素P450含量明显下降,而12～24h后,又明显上升,最高时为对照的1.86倍。
In order to investigate the effect of chlorfenapyr on the biochemical metabolism of beet armyworm larvae and the synergistic potential of the enzyme inhibitor to chlorfenapyr, the toxicity of chlorfenapyr to 3rd, 4th, 5th instar larvae of beet armyworm And synergistic effect of dibutyl maleate (DEM), PBO and TPP on ciclofenac were also investigated. The biochemical analysis was used to determine the inhibitory effect of insecticides Effects of protective enzymes and detoxification enzymes in beet armyworm. The results showed that there was no significant difference in the toxicity of cicladenil to beet armyworm at different instar larvae, and LD50 was basically the same at 24h and 48h after treatment. PBO had antagonistic effect on chlorfenapyr and its synergistic ratio was 0.21, while DEM and TPP had no synergistic effect on chlorfenapyr. Ciclofenac could activate superoxide dismutase (SOD) of beet armyworm larvae and inhibit the activity of catalase, superoxide dismutase and carboxylesterase first. After ciclofenac treatment 6 ~ 24h , Glutathione-S-transferase activity increased significantly, up to 1.85 times the control; treated 6h, the content of cytochrome P450 Beet armyworm decreased significantly from 12 to 24 hours, then increased significantly, the highest control 1.86 times.