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在压力蒸汽灭菌中常用嗜热脂肪杆菌芽胞菌片作为灭菌效果的监测。工作中发现,经细菌培养后有菌生长,而溴甲酚紫蛋白胨水却变色不明显,为此,进行了指示剂的选择试验。所试指示剂有3:①溴甲酚紫,每百毫升蛋白胨水加2％溴甲酚紫乙醇液0.05ml;②酚红,每百毫升蛋白胨水加0.5％酚红溶液0.3ml;③溴麝香草酚蓝,每百毫升蛋白胨水加0.4％溴麝香草酚蓝0.3ml。
In the steam autoclave commonly used lipase Bacillus calmette as a sterilization effect monitoring. Work found that bacteria grew after bacterial growth, but bromocresol peptone water but not obvious discoloration, therefore, conducted a selection test indicator. The test indicators are 3: ① bromocresol purple, peptone water per 100 ml 2% bromocresol purple ethanol 0.05ml; ② phenol red, peptone water per 100 ml 0.5% phenol red solution 0.3ml; ③ bromine Thymol blue, peptone water per 100 ml 0.4% bromothymol blue 0.3ml.