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提出一种化学发光探针介导的声动力学肿瘤成像方法.声敏剂选择性地在肿瘤组织中分布后,通过超声的敏化作用在肿瘤组织中产生活性氧自由基;利用活性氧自由基选择性化学发光试剂直接实时地把声敏化过程中的活性氧自由基转化为光子辐射出来,然后利用光学检测技术进行肿瘤的在体成像.在体外实验中,利用可在体使用的化学发光探针FCLA,对两种声敏剂ATX-70和HpD声动力学反应过程中活性氧自由基的产生进行了直接的实时检测.在移植了人体肿瘤的裸鼠实验中,利用这种方法获得了非常清晰的在体诊断图像.此方法可望在肿瘤的早期临床诊断中得到应用.
A chemoluminescence probe-mediated sonodynamic tumor imaging method was proposed. After selective distribution of the sonosensitizer in the tumor tissue, sensitization of the ultrasound produces reactive oxygen species in the tumor tissue; The base-selective chemiluminescence reagent converts active oxygen radicals in the process of sound sensitization to photon radiation directly in real time, and then utilizes optical detection technology for in vivo imaging of tumors. In vitro experiments utilize in-use chemistry The luminescent probe FCLA detects the production of reactive oxygen species during the acoustic dynamic reaction of the two acoustic sensitizers ATX-70 and HpD in real time. This experiment was performed in a nude mouse transplanted with human tumors. Obtained a very clear in vivo diagnostic image. This method is expected to be applied in the early clinical diagnosis of tumors.