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由于CTMAB或Zeph的引入,Zn(Ⅱ)与7-碘-8-羟基喹啉-5-磺酸(7-I-H_2QS)荧光配合反应被明显地增敏了,荧光强度净增三倍多。主要原因是荧光量子效率的增大。λEX_(max),λEM_(max)和适宜酸度范围由二元体系的373nm,545nm,和pH6.8—7.4,分别移至396nm,526nm和pH3.8—4.8(CTMAB)或pH5.8—6.6(Zeph)。采用7-I-H_2QS作为光试剂,由于分子内重原子效应,使荧光强度和量子效率均比相应的H_2QS体系低,但适宜酸度范围明显地酸移,空白值很低。利用等吸点激发-校正发射谱积分面积比法测定了各体系的荧光量子效率。
Fluorescence complexation of Zn (Ⅱ) with 7-iodo-8-hydroxyquinoline-5-sulfonic acid (7-I-H 2 QS) was sensitized significantly due to the introduction of CTMAB or Zeph with a net increase of more than three times . The main reason is the increase of fluorescence quantum efficiency. The λEX max, λEM max and the appropriate acidity range were shifted from the 373 nm, 545 nm, and pH 6.8-7.4 of the binary system to 396 nm, 526 nm and pH 3.8-4.8 (CTMAB) or pH 5.8-6.6 (Zeph). Using 7-I-H 2 QS as a light reagent, the fluorescence intensity and quantum efficiency are both lower than that of the corresponding H 2 QS system due to the intramolecular heavy atom effect, but the suitable acidity range is obviously acid-shifted and the blank value is very low. The fluorescence quantum efficiency of each system was determined by using the isostimulatory excitation-emission emission spectrum integral area ratio method.