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目的 :寻找肿瘤细胞特异性结合肽 ,为肿瘤诊断试剂及肿瘤药物的研制奠定基础。方法 :用培养的人源肿瘤细胞使裸鼠致瘤 ,将随机肽库从裸鼠的尾静脉注射到裸鼠体内 ,循环数分钟后取肿瘤组织及正常对照组织进行噬菌体筛选 ;经过 2～ 3轮体内筛选 ,获得在肿瘤组织中富集的肽段。结果与结论 :利用体内噬菌体展示技术 ,结合人源肿瘤细胞 (SMMC_772 1和PLA_80 1D)裸鼠致瘤模型 ,从随机十二肽库中成功地筛选到可以较高特异性地结合SMMC_772 1肿瘤细胞或肿瘤血管的噬菌体多肽 ,通过对噬菌体单克隆进行测序 ,初步确定了几种可能的多肽基序(motif)。
Objective: To find tumor cell-specific binding peptides, and to lay the foundation for the development of tumor diagnostic reagents and oncology drugs. Methods: Nude mice were induced to tumor by cultured human tumor cells. The random peptide library was injected into nude mice from the tail vein of nude mice. After several minutes, the tumor tissues and normal control tissues were taken for phage screening. After 2 ~ 3 In vivo screening of the wheel to obtain peptides enriched in tumor tissue. RESULTS AND CONCLUSION: The in vivo phage display technique was used to combine with tumor-inducing model of human tumor cells (SMMC_772 1 and PLA_80 1D) to successfully screen SMMC-7721 tumor cells Or tumor vasculature, several possible polypeptide motifs were initially identified by sequencing the phage monoclonal.