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目的探讨β1肾上腺素能受体激动剂地诺帕明、β2肾上腺素能受体激动剂特布他林、β3肾上腺素能受体激动剂BRL37344对离体大鼠肺泡液体清除率(AFC)的作用及机制。方法5%白蛋白等渗生理盐水溶液和不同药物混合后灌注到离体大鼠的肺泡腔内,根据灌注前及其孵育1h后白蛋白浓度的变化来计算大鼠AFC。结果基础AFC为6.9%±2.2%,地诺帕明、特布他林、BRL37344可显著提高AFC(分别为17.1%±2.4%、19.5%±1.2%、19.9%±2.5%)。β1肾上腺素能受体阻滞剂Atenolol可完全抑制地诺帕明提高AFC(6.1%±0.9%)的作用,但不能阻滞特布他林和BRL37344的作用。β2肾上腺素能受体阻滞剂ICI118551完全抑制了特布他林和BRL37344提高AFC(分别为5.7%±0.6%和7.8%±2.6%)的作用,部分抑制了地诺帕明的作用(AFC为12.7%±1.8%)。β3肾上腺素能受体阻滞剂SR59230A部分抑制了BRL37344和特布他林的作用(AFC分别为13.8%±3.1%和14.5%±3.4%),但不能阻滞地诺帕明的作用。结论地诺帕明、特布他林、BRL37344可以显著提高大鼠的AFC。但地诺帕明和特布他林是分别通过β1、β2肾上腺素能受体起作用;而BRL37344可能是通过β2肾上腺素能受体调节的。ICI118551和SR59230A可能分别具有抑制β1或β2肾上腺素能受体的作用。
Objective To investigate the effect of inhalation of β1 adrenergic receptor agonist dinopamine, β2 adrenergic receptor agonist terbutaline and β37 adrenergic receptor agonist BRL37344 on alveolar fluid clearance (AFC) in isolated rat Role and mechanism. Methods 5% albumin isotonic saline solution and different drugs were mixed into the alveolar space of isolated rat, and the AFC was calculated according to the albumin concentration before perfusion and after 1h incubation. Results AFC was 6.9% ± 2.2%. Adenocarmine, terbutaline and BRL37344 significantly increased AFC (17.1% ± 2.4%, 19.5% ± 1.2%, 19.9% ± 2.5%, respectively). β-1 adrenergic receptor blocker Atenolol completely inhibits the effect of denoplactin on AFC (6.1% ± 0.9%) but does not block the effects of terbutaline and BRL37344. ICI118551, a beta2 adrenergic receptor blocker, completely inhibited the effects of terbutaline and BRL37344 on increasing AFC (5.7% ± 0.6% and 7.8% ± 2.6%, respectively), partially suppressing the effects of dinopamine (AFC 12.7% ± 1.8%). SR59230A, a β3 adrenergic receptor blocker, partially inhibited the effects of BRL37344 and terbutaline (AFC 13.8% ± 3.1% and 14.5% ± 3.4%, respectively), but failed to block the effects of dNaplatin. CONCLUSIONDenopamil, terbutaline and BRL37344 can significantly improve AFC in rats. However, dinapramine and terbutaline act through β1, β2 adrenergic receptors, respectively, whereas BRL37344 is probably regulated by β2 adrenergic receptors. ICI118551 and SR59230A may have the effect of inhibiting [beta] 1 or [beta] 2 adrenergic receptors, respectively.