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目的:探讨恶性肿瘤患者外周血 T 淋巴细胞亚群及其核仁组成区酸性组蛋白表达活性的关系与临床意义。方法:采用 KL-Ⅱ型计算机免疫图象分析系统及碱性磷酸酶抗碱性磷酸酶(APAAP 法)对391例恶性肿瘤、223例良性肿瘤、216例正常人外周血 T 淋巴细胞亚群及其 Ag-NORs 分别进行检测。结果:恶性肿瘤患者CD3、CD4降低,CD8升高,CD4/CD8比值降低。与正常组和良性肿瘤组比较具有显著性差异,其在大肠癌、淋巴瘤中体现特别明显,构成了恶性肿瘤 T 细胞亚群的特点;恶性肿瘤 T 淋巴细胞 I.S%均值与正常组或良性肿瘤组比较,具有显著统计学意义(P 均小于0.01);而良性肿瘤组与正常组比较,无显著差异。结论:恶性肿瘤患者普遍存在T细胞免疫功能低下,T 淋巴细胞 AgNORs 检测可作为肿瘤普查的一种新方法,有助于临床良、恶性肿瘤的鉴别诊断,估计预后,判断疗效。
Objective: To investigate the relationship and clinical significance of peripheral blood T-lymphocyte subsets and the acidic histone expression activity in the nucleolar component of patients with malignant tumors. METHODS: KL-II computerized immunoassay system and alkaline phosphatase anti-alkaline phosphatase (APAAP method) were used in 391 cases of malignant tumors, 223 cases of benign tumors, and 216 cases of normal peripheral blood T lymphocyte subsets. Its Ag-NORs were tested separately. Results: In patients with malignant tumors, the levels of CD3 and CD4 decreased, CD8 increased, and the ratio of CD4/CD8 decreased. Compared with the normal group and the benign tumor group, there is a significant difference, which is particularly evident in colorectal cancer and lymphoma, which constitutes the characteristics of malignant T-cell subpopulations; the mean IS of malignant T-lymphocytes and normal group or benign tumors There was a statistically significant difference between the groups (all P < 0.01). There was no significant difference between the benign tumor group and the normal group. Conclusion: The prevalence of T-cell immune function is common in patients with malignant tumors, and the detection of T-lymphocyte AgNORs can be used as a new method for tumor screening. It is helpful for the differential diagnosis of clinical benign and malignant tumors, the prognosis, and the judgment of efficacy.