论文部分内容阅读
目的 明确喉部良、恶性肿瘤与人类乳头状瘤病毒 (HPV)之间的关系。方法 采用型特异性聚合酶反应(PCR)技术 ,对 6 0例喉癌、36例喉乳头状瘤及 78例声带息肉的新鲜冰冻组织标本进行HPV DNA的检测。结果 HPV 16型阳性者在喉癌组为 2 0例 (33 .33% ) ,喉乳头状瘤组为 4例 (11.11% )及声带息肉组为 4例 (5 .13 % ) (P <0 .0 0 5 ) ;HPV 6 /11型阳性者在喉乳头状瘤组为 35例 (97.2 2 % ) ,喉癌组为 14例 (2 3.33% )及声带息肉组为 16例(2 0 .5 1% ) (P <0 .0 0 5 )。HPV16型感染与喉癌的生物学行为不相关 (P >0 .0 5 ) ;HPV 16型阳性者在吸烟患者中(4 0 % )明显高于非吸烟喉癌患者 (13.33% ) (P <0 .0 5 )。同时还发现 2例恶变的喉乳头状瘤患者均有吸烟史和检出HPV 16型。结论 HPV分型与喉部疾病的良、恶性有关 ;HPV 16型感染与吸烟是喉癌发病学中不容忽视的致癌因素 ;HPV分型的检测对判断喉乳头状瘤的转归有意义。
Objective To clarify the relationship between benign and malignant tumors of the larynx and human papillomavirus (HPV). Methods HPV DNA was detected by PCR in 60 fresh laryngeal carcinomas, 36 laryngeal papillomas and 78 vocal cord polyps. Results HPV16 positive cases were found in 20 cases (33.33%) in laryngeal cancer group, 4 cases (11.11%) in laryngeal papilloma group and 4 cases (5.13%) in vocal cord polyp group (P <0 . There was 35 cases (97.2%) in HPV-positive group, 14 cases (2.33%) in laryngocarcinoma group and 16 cases (20 cases) in vocal cord polyp group. 5 1%) (P <0.05). The HPV16 infection was not related to the biological behavior of laryngeal carcinoma (P> 0.05). HPV16 positive patients were significantly higher than those of non-smoking laryngeal cancer patients (40%) (P < 0 .0 5). Also found that 2 cases of malignant laryngeal papilloma patients have a history of smoking and HPV16 type detected. Conclusion HPV typing is associated with benign and malignant throat diseases. HPV 16 infection and smoking are indispensable carcinogens in the pathogenesis of laryngeal cancer. The detection of HPV genotypes is of great value in judging the prognosis of laryngeal papilloma.