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目的:通过观察组蛋白去乙酰化酶抑制剂(histon-deacetylase inhibitors,HDACIs)丁酸钠(sodium butyrate,NaB)对人乳腺癌细胞株MCF-7细胞的增殖影响以及p27Kip1蛋白表达改变,探讨NaB调控乳腺癌细胞增殖的分子机制。方法:乳腺癌MCF-7细胞经不同浓度NaB作用后,相差显微镜观察细胞形态变化和细胞增殖情况,流式细胞仪分析细胞周期分布,免疫组化检测p27Kip1蛋白表达。结果:NaB对MCF-7细胞有显著的增殖抑制作用,呈时间剂量依赖性,处理后的MCF-7细胞出现凋亡形态变化;细胞周期阻滞于G0/G1期,NaB 2mmol/L组G0/G1期达(62.2±2.2)%,4mmol/L组G0/G1期达(78.1±3.8)%,空白组G0/G1期达(53.1±2.4)%,P<0.05;p27蛋白表达水平上调。结论:NaB可抑制乳腺癌细胞的生长,该作用可能与p27蛋白表达增加有关。
OBJECTIVE: To observe the effects of histone deacetylase inhibitors (sodium butyrate) on the proliferation of human breast cancer cell line MCF-7 and the expression of p27Kip1 protein, and to explore the role of NaB Regulate the molecular mechanism of breast cancer cell proliferation. Methods: The breast cancer cell line MCF-7 was treated with different concentrations of NaB. Cell morphology and cell proliferation were observed by phase contrast microscopy. The cell cycle distribution was analyzed by flow cytometry. The expression of p27Kip1 protein was detected by immunohistochemistry. Results: The proliferation of MCF-7 cells was inhibited by NaB in a time-and dose-dependent manner. The morphological changes of apoptotic MCF-7 cells were observed. The cell cycle arrest was at G0 / G1 phase and the Na 2 mmol / L group / G1 phase was (62.2 ± 2.2)%, the G0 / G1 phase was (78.1 ± 3.8)% in the 4mmol / L group and the G0 / G1 phase was (53.1 ± 2.4)% in the blank group . Conclusion: NaB can inhibit the growth of breast cancer cells, which may be related to the increased expression of p27 protein.