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目的:鉴定引起广豆根根腐病的病原菌,为广豆根根腐病病害的防治提供理论依据。方法:通过组织分离法分离得到病原菌,对其形态特征的观察及该菌rDNA-ITS序列的测定,并与Gen Bank中的序列进行比对。结果:该菌在PDA培养基上菌落圆形,气生菌丝薄绒状,白色,浅灰色,间有土黄色分生孢子座,基物表层肉色。小型分生孢子数量多,卵形、肾形,8～16μm×2.5～4μm;大型分子孢子马特型,多数3～5个隔膜。该菌rDNA-ITS序列长度为553 bp,它与Fusarium solani(登录号为:AB518683.1、AB470903.1、AB369488.1、AJ608989.1、GQ365154.1、EF152426.1)和Fusarium oxysporum(登录号为:GQ922558.1、GQ922559.1、DQ452447.1)的序列同源性均达99%。结论:结合以上两种鉴定方法,得出引起广豆根根腐病的病原菌为半知菌类镰孢霉属真菌茄腐镰孢菌Fusarium solani。
Objective: To identify the pathogen that causes root rot of broad bean and provide a theoretical basis for its prevention and cure. Methods: The pathogen was isolated by tissue isolation method, its morphological characteristics were observed and the rDNA-ITS sequence of the strain was determined and compared with that of Gen Bank. Results: The bacterial colonies were round on PDA medium. The aerial mycelium was thin, velvety, white and light gray with khaki conidia and flesh color on the surface. Small conidia number, ovate, kidney-shaped, 8 ~ 16μm × 2.5 ~ 4μm; large spore Matt type, the majority of 3 to 5 membrane. The rDNA-ITS sequence of this strain was 553 bp in length, which shared the same sequence with Fusarium solani (accession number: AB518683.1, AB470903.1, AB369488.1, AJ608989.1, GQ365154.1, EF152426.1) and Fusarium oxysporum As follows: GQ922558.1, GQ922559.1, DQ452447.1) all had 99% sequence homology. Conclusion: Combining the above two identification methods, it is concluded that the pathogen causing root rot of broad bean is Fusarium solani, a fungus of the genus Fusarium.