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目的 探讨复方鳖甲软肝方 (FFBJRGF)药物血清对离体肝星状细胞 (HSC)细胞周期及凋亡的干预作用。方法 运用流式细胞仪技术检测外源性TGF β1对HSC细胞周期及凋亡率的影响 ,进而观察FFBJRGF高、中、低剂量药物血清对HSC细胞周期及凋亡的干预作用 ,并以IFN γ药物血清及正常对照大鼠血清组作对照。结果 FFBJRGF各剂量药物血清组、TGF β1及IFN γ血清均不同程度增加G0 、G1期HSC细胞数量 ,其中FFBJRGF各剂量药物血清组作用尤为明显 ;与正常对照组比较 ,FFBJRGF各剂量药物血清组对HSCG2 、M、S期有一定的干预作用 ;TGF β1组能显著上调HSC凋亡率 ,但FFBJRGF各剂量药物血清组及IFN γ血清组作用不明显。结论 FFB JRGF药物血清具有显著抑制HSC细胞增殖周期作用 ,可能为其抗肝纤维化的机制之一 ,但对体外HSC细胞凋亡影响作用不明显
Objective To investigate the intervention effect of FFBJRGF drug serum on cell cycle and apoptosis of isolated hepatic stellate cells (HSC). Methods Flow cytometry was used to detect the effect of exogenous TGF-β1 on the cell cycle and apoptosis rate of HSCs. Then the effects of high, middle and low doses of FFBJRGF on the cell cycle and apoptosis of HSC were observed, and IFN-γ was observed. Drug serum and normal control rat serum group were used as controls. Results The serum levels of FFBJRGF, TGFβ1, and IFNγ all increased the number of HSC and G1 phase HSC cells in different degrees. Among them, the FFBJRGF doses in each drug serum group were particularly effective. Compared with the normal control group, the FFBJRGF doses in each drug group were comparable. HSCG2, M and S phases had a certain intervention effect; TGFβ1 group could significantly upregulate the apoptotic rate of HSC, but FFBJRGF had no obvious effect on serum doses of IFN-γ serum and IFN-γ serum. Conclusion FFB JRGF drug serum has a significant inhibitory effect on the proliferation cycle of HSC cells, which may be one of the mechanisms of anti-hepatic fibrosis, but it has no obvious effect on the apoptosis of HSC cells in vitro.