疟原虫的许多虫种不能只根据红内期形态来鉴别。rRNA,特别是SSUrRNA被认为是鉴别疟原虫的理想靶分子。该分子由保守区和可变区嵌合而成,利用疟原虫属共有的保守区作引物就可使各种疟原虫的序列得以扩增。经PCR扩增产生的片段与已知的寡核苷酸探针杂交或作序列分析即可鉴定虫种。扩增寄生虫RNA时有竞争机制,得到的产物与原样本中靶物量的比例相平行;这种反 Many species of Plasmodium can not be identified based solely on the red stage morphology. rRNAs, and in particular SSUrRNAs, are considered to be ideal targets for the identification of Plasmodium. The molecule consists of a conserved region and a variable region chimeric. Using the conserved region common to Plasmodium as a primer, the sequence of various plasmodium species can be amplified. The resulting species can be identified by hybridization or sequence analysis with known oligonucleotide probes. There is a competitive mechanism when amplifying parasite RNA, and the resulting product is parallel to the ratio of target amount in the original sample; this reverse