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目的探讨 RCAS1与雌激素受体亚型在正常子宫内膜、增生症内膜和内膜癌组织中的表达及相关性,分析它们在内膜癌发生发展中的作用。方法采用免疫组化、RT-PCR 法检测20例正常子宫内膜组织、42例子宫内膜增生症组织及50例内膜癌组织中 RCAS1和雌激素受体α(ERα)雌激素受体β(ERβ)的表达,免疫印迹法检测各组内膜中 RCAS1蛋白的表达。结果免疫组化显示RCAS1在正常、简单型和复杂型增生内膜中均为 P 表达模式,不典型增生内膜中30%(6/20)为 D 表达模式,内膜癌中均为 D 模式。正常内膜、简单型和复杂型增生、不典型增生、内膜癌 RCAS1蛋白高水平表达率分别为0%(0/20),9.1%(2/22),45.0%(9/20),68.0%(34/50),各组间差异有统计学意义(P<0.05)。RCAS1蛋白高表达与患者肌层侵犯深度、脉管侵犯、ERα阳性相关(P<0.05)。免疫印迹法、RT-PCR 结果与免疫组化结果一致。RCAS1 mRNA 的表达水平与 ERα mRNA 呈显著正相关,而与 ERB mRNA 表达未显现相关性。结论 RCAS1高表达与表达模式的改变可能参与子宫内膜从良性到恶性转变的过程,RCAS1协同 ERα表达与子宫内膜癌的发展、浸润转移有关。
Objective To investigate the expression and correlation of RCAS1 and estrogen receptor subtypes in normal endometrium, endometrial hyperplasia and endometrial carcinoma and their roles in the carcinogenesis and progression of endometrial carcinoma. Methods Immunohistochemistry and RT-PCR were used to detect the expressions of RCAS1 and ERα in 20 normal endometrium tissues, 42 endometrial hyperplasia tissues and 50 endometrial carcinoma tissues. (ERβ), Western blotting was used to detect the expression of RCAS1 protein in each group. Results Immunohistochemistry showed that RCAS1 was P-type expression in normal, simple and complex proliferative endometrium, D (30%) in atypical hyperplastic endometrium and D mode in endometrial carcinoma . The high expression rates of RCAS1 protein in normal endometrium, simple and complex hyperplasia, dysplasia and endometrial carcinoma were 0% (0/20), 9.1% (2/22) and 45.0% (9/20), respectively 68.0% (34/50), the difference between each group was statistically significant (P <0.05). The high expression of RCAS1 protein was associated with muscular layer invasion, vascular invasion and ERα positive (P <0.05). Western blotting, RT-PCR results and immunohistochemistry results. The expression of RCAS1 mRNA was positively correlated with ERα mRNA, but not with ERB mRNA expression. Conclusion The changes of RCAS1 expression and expression may be involved in the process of benign to malignant transformation of endometrium. The expression of RCAS1 in combination with ERα may be related to the development of endometrial carcinoma and invasion and metastasis.