根据Jerne的独特型-抗独特型网络理论,本文研究用单克隆Anti-Id代替常规检测中的HBsAg,建立一种新的检测抗-HBs的方法。该法用Anti-Id作为包被抗体,HRP标记的羊抗人IgG检测被检血清中被结合的抗-HBs。实验结果表明,本法具有良好的敏感性(0.5μg/ml)和特异性,而且安全、简便,试剂来源丰富,尤其适用于检测那些抗原不易获得,不易纯化或抗原传染性强的疾病。 According to Jerne’s unique type-anti-idiotype network theory, a new method of detecting anti-HBs was established by replacing the conventional HBsAg with monoclonal anti-Id. This method uses Anti-Id as a coated antibody, and HRP-labeled goat anti-human IgG detects bound anti-HBs in sera. The experimental results show that this method has good sensitivity (0.5μg / ml) and specificity, and is safe, simple and rich source of reagents, especially for the detection of those diseases which antigen is not easy to obtain, difficult to be purified or antigen highly infectious.