我市大毛山、未里庄等大队,采用“砂种法”生产“5046”。这种方法,简便易行,节省培养时间,不易污染,节省燃料和原料。具体方法是: 一、取细砂过筛,用清水洗涤数次,不再混浊为止。然后用锅炒,温度达到150℃以上后保持十分钟,铲出后放在洁净的磁面盆或罐头瓶内冷凉备用。二、取一清洁大碗和汤匙,倒人少许酒精擦拭,剩余部分点燃进行灭菌,凉后倒入半斤左右无菌砂,在无菌条件下,将3～4支(160×16或180×18毫米的试管)斜面菌苔 Da Mao Shan City, not the village and other brigade, the use of “sand species” production “5046.” This method, simple and easy, save training time, not easy to pollution, save fuel and raw materials. The specific method is: First, take fine sand sieved, washed with water several times, no longer cloudy. Then use the pan fried, the temperature reached above 150 ℃ for 10 minutes after shoveling out on a clean magnetic basin or cans bottle cool standby. Second, take a clean bowl and spoon, pour a little alcohol wipe, the remaining part of the lit sterilization, pour about half a catty cool sterile sand, under sterile conditions, the 3 to 4 (160 × 16 or 180 × 18 mm tube) beveled bacteria