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目的:对比研究经静脉移植骨髓间充质干细胞(MSCs)及骨髓间充质干细胞联合中药移植对心肌梗死兔的血清P-selection(P-选择素)的影响,推测干细胞移植的安全性,以及药物干预骨髓间充质干细胞移植对心功能的影响。方法:取兔骨髓间充质干细胞进行体外培养、增殖和标记,同时将已建立心肌梗死模型的日本大耳白兔随机分为4组,模型对照组(B组),静脉0天注射干细胞组(C组)、静脉0天注射丹参组(D组)、静脉0天注射丹参联合干细胞组(E组),开胸结扎冠状动脉前降支的方法建立急性心肌梗死模型,造膜即刻经静脉注射已经分离、培养、扩增、标记后的MSCs悬液,静脉注射中药组经静脉注射丹参注射液。移植后24h检测外周血血清肌酸激酶同功酶(CK-MB),移植后24h及5周检测外周血中P-selection含量,探讨干预因素对内皮功能的影响。移植后4周测定心功能。结果:超声检查显示,与A组相比,B、C、D各组心功能明显降低,差异有统计学意义;与B组相比,E组心功能明显改善,差异有统计学意义,C、D组虽有心功能改善,但不具统计学意义,C组与D组间无统计学差异。血清P-选择素检测示:与B组比较,C、D、E各组均有明显的降低,差异有统计学意义;C、D、E组间无统计学差异。结论:中药丹参可促进MSCs移植治疗心肌梗死的作用,可有效降低P-选择素的表达,有效的改善心肌梗死兔的心功能。
OBJECTIVE: To compare the effects of intravenous transplantation of bone marrow mesenchymal stem cells (MSCs) and bone marrow mesenchymal stem cells (MMSCs) combined with traditional Chinese medicine on serum P-selection (P-selectin) in rabbits with myocardial infarction and to evaluate the safety of stem cell transplantation Influence of Drug Intervention on Cardiac Function after Bone Marrow Mesenchymal Stem Cell Transplantation. Methods: Rabbit bone marrow-derived mesenchymal stem cells were cultured, proliferated and labeled in vitro. At the same time, Japanese white rabbits with established myocardial infarction model were randomly divided into 4 groups: model control group (B group), intravenous 0-day injection of stem cell group (Group C). Danshen group (group D) was injected intravenously on day 0, group D received intravenous injection of Danshen combined with stem cells (group E), and thoracotomy for ligation of the anterior descending coronary artery was used to establish acute myocardial infarction model. After the injection, the MSCs suspension that has been isolated, cultured, amplified and labeled is intravenously injected into the Chinese traditional medicine group and the Danshen injection is intravenously injected. Peripheral blood creatine kinase isoenzyme (CK-MB) was detected 24 h after transplantation, P-selection in peripheral blood was measured 24h and 5 weeks after transplantation, and the effect of intervention on endothelial function was also investigated. Four weeks after transplantation, cardiac function was measured. Results: Compared with group A, the cardiac function of group B, C and D was significantly lower than that of group A, and the difference was statistically significant. Compared with group B, the cardiac function of group E was significantly improved, the difference was statistically significant. C Although D group had improvement of cardiac function, but not statistically significant, there was no significant difference between C group and D group. Serum P-selectin test showed: Compared with B group, C, D, E groups were significantly reduced, the difference was statistically significant; C, D, E group was no significant difference. Conclusion: Salvia miltiorrhiza can promote MSCs transplantation in the treatment of myocardial infarction, which can effectively reduce the expression of P-selectin and effectively improve the cardiac function in rabbits with myocardial infarction.