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在一套由搅拌罐和管式反应器串联而成的组合式反应系统中,利用酿酒酵母进行连续发酵生产高浓度乙醇。后续管式反应器内通过装填聚氨酯颗粒和木块对酵母细胞进行吸附固定化,在乙醇抑制造成细胞活性大幅降低的情况下,通过大幅提高细胞浓度保证发酵效率,在稀释速率0.02h-1和280g/L葡萄糖的条件下,系统的终点乙醇浓度为15.4%(v/v)。研究表明在一定稀释速率之下,应该通过增加反应器的级数来降低稀释速率,以达到提高终点乙醇浓度,如简单地降低进料速率则可能增加整个系统所受的乙醇抑制,对提高终点乙醇浓度效果不显著。
In a combined reaction system consisting of a stirred tank and a tubular reactor, continuous fermentation is carried out using Saccharomyces cerevisiae to produce high-concentration ethanol. Subsequent tubular reactor through the filling of polyurethane particles and wood pieces of yeast cells were immobilized in the ethanol inhibition caused by a significant reduction in cell activity by drastically increasing the cell concentration to ensure the fermentation efficiency in the dilution rate of 0.02h-1 and The final ethanol concentration was 15.4% (v / v) at 280 g / L glucose. Studies have shown that at a certain dilution rate, the dilution rate should be decreased by increasing the reactor stages to achieve an increase in endpoint ethanol concentration. Simply lowering the feed rate may increase the overall ethanol inhibition, Ethanol concentration effect is not significant.