论文部分内容阅读
目的:探索受精失败卵子纺锤体、染色体的立体结构变化及其相关因素。方法:选自本所受精卵子的材料分两组实验组:卵子来源于IVF取卵后24～40 h受精失败的MⅡ期卵子。对照组:为未授精卵子。两组卵子通过免疫荧光染片后,用Nikon激光共聚焦显微镜观察染色体、纺锤体的立体结构,根据年龄段、促排卵药的差别进行分组,用t和x~2检验进行统计学分析。结果:年龄小于30岁组的纺锤体、染色体正常率分别为:13.3%、13.3%,与对照组相比差异均无显著性,(P>0.05)。30～35岁组的纺锤体、染色体正常率分别为:16%,12%,纺锤体正常率与对照组差异相比,无显著性,(P>0.05),染色体正常率与对照组相比,差异有显著性,(P<0.05)。年龄大于35岁组的纺锤体、染色体正常率分别为:0%、0%,与对照组相比均差异有显著性,(P<0.05)。合计年龄小于35岁患者受精失败卵子的纺锤体、染色体正常率分别为:15%、12.5%,与对照组比,无显著性差异。果纳芬组的纺锤体及染色体的正常率均为12.5%,与对照相比,无显著性差异,(p>0.05)。丽申宝组纺锤体及染色体的正常率分别为11.1%,5.6%,纺锤体正常率与对照组相比,差异无显著性,(P>0.05),染色体正常率与对照组相比,差异有显著性,(P<0.05)。实验组的总的纺锤体和染色体正常率为12%、10%,与对照组相比均差异有显著性,(P<0.05)。结论:高龄妇女受精失败卵子的纺锤体和染色体的正常率下降;用丽申宝进行超促排卵的受精失败卵子染色体正常率下降;染色体非整体倍增加的一个相关因素,可能对纺锤体正常梭形结构的破坏。
Objective: To explore the three-dimensional structural changes of chromosomes and related factors in fertilized egg spindles. Methods: Materials selected from our fertilized eggs were divided into two groups. The eggs were derived from the M Ⅱ oocytes that had failed to fertilize 24 ~ 40 h after IVF retrieval. Control group: non-fertilized eggs. Two groups of eggs through the immunofluorescence staining, Nikon confocal laser scanning microscopy of chromosomes, spindle three-dimensional structure, according to the age group, ovulation induction differences were grouped by t and x ~ 2 test for statistical analysis. Results: The chromosomes of the spindles aged less than 30 years were 13.3% and 13.3% respectively, which showed no significant difference compared with the control group (P> 0.05). The normal rate of spindle and chromosome in 30-35 years old group was 16% and 12%, respectively. There was no significant difference between the normal rate of spindle and the control group (P> 0.05). The chromosome normal rate was significantly higher than that of the control group , The difference was significant (P <0.05). The chromosomes of the spindles aged more than 35 years were 0% and 0%, respectively. The difference was significant compared with the control group (P <0.05). Totally less than 35-year-old patients fertilized eggs failed to spindles, chromosome normal rates were: 15%, 12.5%, compared with the control group, no significant difference. The normal rate of spindle and chromosome in the fruit satisfied group was 12.5%, no significant difference compared with the control (p> 0.05). The normal rates of spindles and chromosomes in Lishenbao group were 11.1% and 5.6%, respectively. There was no significant difference in spindle normal rate between the two groups (P> 0.05). The difference of chromosomal normal rate There was significant (P <0.05). The total spindle and chromosome normal rates in the experimental group were 12% and 10%, respectively, which were significantly different from those in the control group (P <0.05). CONCLUSION: The normal rate of spindles and chromosomes of fertile failed fertilized eggs decreased in elderly women. The fertilization failure of ovipositor failed to reduce the normal rate of chromosomal abnormality of the egg. A related factor of chromosome non-integral doubling may affect the spindle normal shuttle Destruction of the shape structure.