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目的通过在麻醉不同时点对手术环境致病微生物进行检验,评估不同麻醉时点的微生物感染风险,为控制患者医院感染的发生提供参考依据。方法选择我院手术室全麻相关器材进行采样,采样对象为静脉三通旋转阀、氧气流量调节钮、螺纹管接口、麻醉机呼出气入口和麻醉机呼吸回路氧气入口。选择每日各手术室第1例全麻手术且预计手术时间≥2 h者进行采样,共采集了75例手术的375个采样点,并进行采样和细菌培养检测。结果本次采集的75例手术375个采样点标本检测后发现,静脉三通旋转阀在时点1的标本未培养出细菌,合格率为100%,时点2检出阳性57例,合格率为24.0%,时点3检出阳性75例,合格率为0.0%;氧气流量调节钮、螺纹管接口、麻醉机呼出气入口以及麻醉机呼吸回路氧气入口按照相关标准各时点的合格率均为100%。结论手术麻醉过程中,麻醉相关器械容易造成污染,尤其静脉三通旋转阀,应当在麻醉过程中严格执行无菌操作规范,控制因麻醉过程引起的医院感染。
Objective To evaluate the risk of microbial infection at different anesthesia points by testing the pathogenic microorganisms in surgical environment at different time points of anesthesia and provide reference for controlling the occurrence of nosocomial infections in patients. Methods The general anesthesia equipment in our operating room was selected for sampling. The objects of sampling were venous three-way rotary valve, oxygen flow control button, threaded tube interface, anesthetic machine exhaled air inlet and anesthetic machine breathing circuit oxygen inlet. Select the first day of each operating room anesthesia surgery and the estimated operation time of 2 h were sampled, a total of 75 cases were collected 375 sampling points, and sampling and bacterial culture testing. Results The 75 samples collected from 75 surgical specimens were collected and tested. The results showed that no bacteria were cultured in the sample at time 1, the pass rate was 100%, the positive rate was 57, and the pass rate 24.0%, the time point 3 positive 75 cases, the pass rate was 0.0%; oxygen flow control button, threaded tube interface, anesthesia machine exhaled air inlet and anesthesia machine oxygen breathing circuit entrance at all times according to the relevant standards pass rate It is 100%. Conclusion During anesthesia, anesthesia-related instruments are easy to cause pollution. In particular, intravenous three-way rotary valves should strictly implement aseptic practices in anesthesia to control nosocomial infections caused by anesthesia.