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为研究喉癌与人乳头状瘤病毒(HPV)感染的关系,本研究探讨了HPV在喉癌中的致病作用和基因组型的分布与表达。应用共有引物和多重引物PCR的方法,对160例喉不同病变的新鲜组织标本,进行HPV6、11、16、18、31、33、35、42、58共9型HPVsDNAs感染的检测。结果在喉癌组HPV感染的阳性率为49.3％(35/71),喉癌颈转移淋巴结组为22.7％(5/22),喉癌前病变组为11.8％(2/17),声带息肉组为6.7％(2/30),癌周正常喉组织为0％(0/20)。HPV DNA型别分布在喉癌中以HPV16、18型为主,喉良性病变中以HPV6、11型为主。表明喉癌发生发展与HPV感染相关。
In order to study the relationship between laryngeal cancer and human papillomavirus (HPV) infection, this study explored the pathogenicity and genomic distribution and expression of HPV in laryngeal cancer. Using common primers and multiple primers PCR, HPV16, HPV16, HPV11, HPV18, HPV16, HPV18, HPV31, HPV31, HPV43, HPV43 and HPV58 were detected in 160 fresh tissue specimens of different pathological changes. Results The positive rate of HPV infection was 49.3% (35/71) in laryngeal cancer group, 22.7% (5/22) in cervical laryngeal cervical lymph node group, 11.8% (2/17) in laryngeal precancerous lesion group, The group was 6.7% (2/30), and the normal laryngeal tissue was 0% (0/20). HPV DNA type distribution in laryngeal cancer mainly HPV16, 18, mainly in the type of benign throat HPV6,11. Show that the occurrence and development of laryngeal cancer and HPV infection.