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目的:建立HPLC法测定利胆消石丸中黄芩苷含量的方法。方法:采用Shim-pack VP-ODS色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(47:53:0.2),检测波长280nm,流速1.0ml·min~(-1),柱温加℃。结果:黄芩苷在0.24～1.21μg(r=0.999 9)范围内呈良好的线性关系;平均加样回收率为101.1%,RSD=2.0%(n=6)。结论:该方法简便,准确,可用于利胆消石丸的质量控制。
Objective: To establish a HPLC method for the determination of baicalin in Lidan Xiaoshi Pills. METHODS: A Shim-pack VP-ODS column (150 mm×4.6 mm, 5 μm) was used. The mobile phase was methanol-water-phosphoric acid (47:53:0.2). The detection wavelength was 280 nm and the flow rate was 1.0 ml·min~(-1). ), column temperature plus °C. RESULTS: Baicalin showed a good linear relationship in the range of 0.24 to 1.21 μg (r=0.999 9); the average recovery was 101.1% and the RSD was 2.0% (n=6). Conclusion: This method is simple, accurate, and can be used for the quality control of Lidan Xiaoshi pill.