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目的分析2009-2012年河南省德尔卑沙门菌和阿贡纳沙门菌临床分离株的耐药状况与脉冲场凝胶电泳(PFGE)带型,为以德尔卑沙门菌、阿贡纳沙门菌等非伤寒沙门菌为代表的食源性疾病暴发预警、调查、溯源及公共卫生意义上的抗生素使用策略提供基线与参考数据。方法根据国际PulseNet细菌性传染病分子分型监测网络公布的非伤寒沙门菌PFGE分型技术与美国临床与实验室标准协会(CLSI)沙门菌K-B法药敏测试方案,对2009-2012年分离自河南省5个哨点医院的德尔卑沙门菌与阿贡纳沙门菌进行13种抗生素的药敏测试及PFGE脉冲场凝胶电泳分子分型分析。结果 76株德尔卑沙门菌与84株阿贡纳沙门菌对8类13种抗生素均有不同程度的耐药,耐3种以上抗生素的有141株(88.13%)。其中耐3~5种抗生素38株(占23.75%),耐6~8种抗生素66株(占41.25%),耐9~10种抗生素22株(占13.75%),耐11~12种抗生素15株(占9.38%)。84株阿贡纳沙门菌经XbaⅠ酶切与脉冲场凝胶电泳后获得36种带型,每种带型包含1~9个菌株,相似度50.77%~100%,以AGN27与AGN33为主要优势带型。76株德尔卑沙门菌经XbaⅠ酶切与脉冲场凝胶电泳后获得41种带型,每种带型包含菌株数1~12个菌株,相似度43.25%~100%,以DER17与DER34为主要优势带型。结论河南省临床分离株德尔卑沙门菌和阿贡纳沙门菌耐药状况较严重,PFGE带型呈现出多样性的同时又具有较显著的优势带型特点,部分带型与其对应的耐药谱具有一定的关联性及相同的聚集性。
Objective To analyze the resistance status and the PFGE banding patterns of Salmonella del valleys and Argonauna Salmonella delphi in Henan Province from 2009 to 2012, Non-Salmonella Salmonella represented foodborne disease outbreak warning, investigation, traceability and public health significance of antibiotic strategies to provide baseline and reference data. Methods Based on the PFGE typing of non-typhoid Salmonella PFGE reported by the International PulseNet Network for Molecular Detection of Bacterial Infectious Diseases and the CLSI susceptibility test program of Salmonella in the United States of America from 2009 to 2012, Thirteen antibiotics susceptibility tests and PFGE pulsed-field gel electrophoresis molecular typing analysis of Salmonella del valde and Salmonella typhi were carried out in five sentinel hospitals in Henan Province. Results 76 strains of Salmonella delphi and 84 strains of Salmonella Agaric were resistant to 8 kinds of 13 kinds of antibiotics, and 141 strains (88.13%) were resistant to more than 3 kinds of antibiotics. Among them, 38 strains were resistant to 3-5 antibiotics (23.75%), 66 strains were resistant to 6-8 antibiotics (41.25%), 22 strains (13.75%) were resistant to 9-10 antibiotics and 11-12 antibiotics Strain (9.38%). 84 strains of Salmonella enteritidis were digested with Xba Ⅰ and pulsed-field gel electrophoresis, and each band contained 1 to 9 strains with a similarity of 50.77% -100%. The main advantages of AGN27 and AGN33 Belt type. 76 strains of Salmonella delphi were digested by Xba Ⅰ and pulsed-field gel electrophoresis, and 41 bands were obtained. Each band contained 1 to 12 strains with the similarity of 43.25% -100%. DER17 and DER34 were the main Advantage belt type. Conclusion The clinical isolates of Salvia miltiorrhiza and Salmonella argona have more serious drug-resistance status in Henan province. PFGE patterns show a diversity and at the same time, they are more dominant bands. Some bands and their corresponding drug resistance spectrum Has a certain relevance and the same aggregation.