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目的采用新型干燥血栓微粒建立兔局灶性脑梗死模型,探讨不同栓塞剂量与梗死部位和梗死程度的关系。方法兔自体血通过自凝、烘干、碾磨和滤网过滤制得干燥血栓微粒。栓塞组兔经右侧颈内动脉注入不同剂量(2.5、5.0、7.5、10.0、12.5、15.0、17.5 mg组)的干燥血栓微粒,对照组兔则注入等量生理盐水,分别在栓塞后2、72 h行神经功能评级,栓塞前及栓塞后72 h检测血浆脑损伤因子S100B含量,栓塞后72 h处死动物取脑组织进行病理学检查。结果干燥血栓微粒大小均一(直径100～150μm),生理盐水稀释后分布均匀、不易成团、不易自溶,栓塞成功率达90.5%。栓塞后72 h,栓塞组血浆S100B水平显著高于栓塞前和对照组,脑组织水含量高于对照组;各栓塞剂量组实验兔的病死率、神经功能评级及脑梗死体积比与栓塞剂量呈正相关;TTC染色脑片显示栓塞2.5、5.0、7.5 mg组80%动物梗死灶位于右侧大脑皮质,而栓塞10.0、12.5、15.0 mg组动物双侧大脑皮质均出现梗死灶。结论利用干燥血栓微粒成功建立兔脑梗死模型,方法简单、成功率高,2.5、5.0、7.5 mg和10.0、12.5、15.0 mg可分别模拟单、双侧脑梗死,为脑梗死的治疗研究提供了不同类型的临床前模型。
Objective To establish a model of focal cerebral infarction in rabbits with a new type of dry thrombus particles and investigate the relationship between different embolization doses and the infarct size and the degree of infarction. Methods Rabbit autologous blood was obtained by self-coagulation, drying, grinding and filter filtration to obtain dry thrombus particles. The rabbits in the embolization group were given different doses (2.5, 5.0, 7.5, 10.0, 12.5, 15.0 and 17.5 mg groups) of dry thrombus particles through the right internal carotid artery. The rabbits in the control group were given the same amount of normal saline, The neurological function was evaluated at 72 h before operation. The levels of S100B in plasma were measured before embolization and at 72 h after embolization. The brain tissues were sacrificed at 72 h after embolization for pathological examination. Results The desiccated thrombus particles were uniform in size (100-150 μm in diameter). The saline solution was evenly distributed after dilution and was not easy to dissolve. The success rate of embolization was 90.5%. At 72 h after embolization, the level of plasma S100B in embolization group was significantly higher than that before embolization and in control group, and the water content in brain tissue was higher than that in control group. The mortality, neurological function rating and cerebral infarction volume ratio in rabbits in each embolization dose group were positive TTC-stained brain slices showed that embolization of 2.5, 5.0, 7.5 mg group 80% of animal infarcts located in the right cerebral cortex, and embolization 10.0,12.5,15.0 mg animals bilateral cerebral cortex were infarction. Conclusion The successful establishment of a rabbit model of cerebral infarction using dry thrombus particles is simple and has a high success rate. 2.5, 5.0, 7.5 mg and 10.0, 12.5 and 15.0 mg can be used to simulate single and bilateral cerebral infarction respectively and provide a therapeutic approach for the treatment of cerebral infarction Different types of preclinical models.