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目的 :探讨祁白术等治疗饮食失节所致脾虚小鼠模型的研究。方法 :模型小鼠随机分脾虚、祁白术、四君子汤、生及炒白术、正常、自然恢复等 7组。治疗前后分别取胰、肝、胃肠等进行组化、透射和扫描电镜观察。结果 :脾虚组胰腺泡上皮酶原、胰岛细胞、胃主及壁细胞、G细胞、内分泌细胞、十二指肠绒毛及吸收上皮微绒毛、小肠粘膜圆顶区 M细胞等均减少。固有膜呈慢性炎症 ,微循环瘀血明显。胰腺泡腔、上皮及胞核内有大肠杆菌感染。十二指肠绒毛及肠腺、肝及胰 ATP酶、SDH酶缺乏 ,肝苏丹 染色为脂肪变 ,肝、肌 PAS糖原染色减低、消失。经祁白术等治疗后上述病变皆有恢复。结论 :祁白术、四君子汤等对脾虚小鼠的糖、脂肪、蛋白质代谢及肠道、全身粘膜系统免疫障碍有复健作用 ,特别是祁白术能使血脂、脂肪肝、血糖降至正常。
Objective: To investigate the treatment of spleen deficiency mice model caused by dietary imbalance. Methods : Model mice were randomly divided into 7 groups: Spleen deficiency, Atractylodes macrocephala, Sijunzi Decoction, Health and Atractylodes Rhizome, normal and natural recovery. Before and after treatment, the pancreas, liver, and gastrointestinal tract were taken for histochemistry, transmission, and scanning electron microscopy. RESULTS: In the spleen deficiency group, the number of pancreatic vesicle epithelial plasmogens, islet cells, gastric lord and parietal cells, G cells, endocrine cells, duodenal villi, absorptive epithelial microvilli and M cells in the small intestine mucosal dome area were all decreased. Intrinsic membrane was chronic inflammation, and microcirculation was obvious. There is E. coli infection in the pancreatic cavity, epithelium and nucleus. Duodenal villus and intestinal glands, liver and pancreas ATPase, SDH enzyme deficiency, liver sulphate staining for fat, liver, muscle PAS glycogen staining decreased, disappeared. After the treatment of Atractylodes macrocephala, these lesions recovered. Conclusion: Atractylodes lucidum, Sijunzi decoction and so on have the rehabilitation effect on the metabolism of sugar, fat, protein and immune system disorders of intestinal and systemic mucosa in spleen deficiency mice. In particular, Atractylodes macrocephala can reduce blood fat, fatty liver and blood sugar to normal.