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目的:建立复方甲硝唑栓中硫酸庆大霉素含量的测定方法。方法:根据硫酸庆大霉素与碱性酒石酸铜试液形成有色复合物这一特性,选择560 nm波长处用比色法测定其含量。结果:硫酸庆大霉素在0.4～1.4万u·ml~(-1)浓度范围内呈良好的线性关系,平均回收率为97.86%,RSD为1.43%。结论:本含量测定方法简便、快速、准确、重复性好,适用于复方甲硝唑栓中硫酸庆大霉素的含量测定。
Objective: To establish a method for the determination of gentamicin sulfate in compound metronidazole suppository. Methods: According to the characteristic of the formation of colored complex between gentamicin sulfate and alkaline tartrate test solution, the content of gentamicin sulfate at 560 nm was determined by colorimetry. Results: Gentamicin sulfate showed a good linearity in the range of 0.4-1.4 000 u · ml -1 with an average recovery of 97.86% and a RSD of 1.43%. Conclusion: The determination of content is simple, rapid, accurate and reproducible. It is suitable for the determination of gentamicin sulfate in compound metronidazole suppository.