The binding of nuclear proteins prepared from mouse erythroid tissue in different developmental stages to the 5’-flanking regulatory elements of human globin gene, two negative control regions(NCR1, -610 to -490 bp; NCR2,-338 to -233bp), was identified. Two stage specific protein factors corresponding to embryonic and fetal stages were found to be capable of binding to NCR2. These data provided evidence that the cis acting elements of the 5’-flanking region might be involved in the developmental control of globin gene and NCR2 might be responsible in part for the silence of globin gene in the embryonic and fetal stages. The binding of nuclear proteins prepared from mouse erythroid tissue in different developmental stages to the 5’-flanking regulatory elements of human globin gene, two negative control regions (NCR1, -610 to -490 bp; NCR2, -338 to -233 bp), was identified. Two data related to embryonic and fetal stages were found to be capable of binding to NCR2. These data provided evidence that the cis acting elements of the 5’-flanking region might be involved in the developmental control of globin gene and NCR2 might be responsible in part for the silence of globin gene in the embryonic and fetal stages.