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目的了解天津市秋冬季腹泻患儿的星状病毒感染情况及其分型特点。方法采用逆转录聚合酶链反应(RTPCR)技术,于2014年9月—2015年2月收集天津市儿童医院住院部1 127例疑似病毒性腹泻的婴幼儿粪便样品中的星状病毒核酸进行检测,并将部分阳性株的PCR产物进行测序,采用序列分析软件分析测序结果,推断其毒株型别,同时收集患儿的临床资料进行综合分析。结果 1 127份腹泻粪便样品中,星状病毒RT-PCR阳性99份,检出率为8.78%。感染星状病毒的患儿多为1岁以下,患儿年龄中位数为3.00月龄,且1月检出率最高。99例阳性星状病毒患儿中,有52例与其他病毒混合感染,混合感染率为4.61%,其中以诺如病毒合并星状病毒最常见。26份PCR阳性产物送往测序,经Genbank Blast及系统进化树分析,所有星状病毒均为人类星状病毒1型。结论星状病毒是天津市秋冬季婴幼儿病毒性腹泻的重要病原体,本次调查期间星状病毒以人类星状病毒1型毒株流行为主,此外存在一定比例的混合感染。
Objective To understand the status of astrovirus infection in children with diarrhea in autumn and winter in Tianjin and its typing characteristics. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the presence of astrovirus in 1 127 infant and feces samples from inpatient department of Tianjin Children’s Hospital from September 2014 to February 2015. , And PCR products of some positive strains were sequenced. Sequence analysis software was used to analyze the sequencing results to infer the strain type, and to collect the clinical data of children for comprehensive analysis. Results Among the 1 127 diarrhea stool samples, 99 were positive for RT-PCR by astrovirus and the detection rate was 8.78%. Children infected with astrovirus mostly less than 1 year old, children with a median age of 3.00 months, and the highest detection rate in January. Of the 99 cases of positive astrovirus, 52 cases were mixed with other viruses, the mixed infection rate was 4.61%. Among them, Norovirus combined with Astrovirus was the most common. Twenty-six PCR positive products were sequenced and analyzed by GenBank and phylogenetic trees. All astroviruses were human astrovirus type 1. Conclusions Astrovirus is an important pathogen of infantile diarrhea in infants and young children in autumn and winter of Tianjin. During the survey, the prevalence of astroviruses was 1, and there was a certain proportion of mixed infections.