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目的通过研究白藜芦醇(resveratrol,Res)对3T3-L1脂肪细胞线粒体生物合成的影响,探讨其在细胞能量代谢中的作用。方法诱导3T3-L1前脂肪细胞分化,分别设置对照组和10、20、40μmol/L的Res干预组,油红O染色观察细胞内脂质蓄积情况,透射电镜观察细胞内线粒体的数量和形态,蛋白免疫印迹法(Western blot)检测线粒体合成过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptorγ,PPARγ)、过氧化物酶体增殖物激活受体共激活因子(peroxisome proliferato-activated receptorγcoactivator-1α,PGC-1α)、含PR结构域的锌指蛋白16(PR domain-containing 16,PRDM16)的表达水平。结果 Res干预导致10、20、40μmol/L组细胞内的脂质蓄积量与对照组相比分别减少了10.7%、38.9%、62.9%(P均<0.01);细胞内的线粒体数量增加,体积增大;细胞内PPARγ、PGC-1α、PRDM16蛋白表达水平增加。其中10μmol/L组PPARγ、PGC-1α的表达量为对照组的(1.91±0.38)、(1.59±0.10)倍(P<0.01),20μmol/L组PPARγ、PGC-1α和PRDM16的表达量为对照组的(3.76±0.28)、(1.61±0.15)和(1.30±0.10)倍(P<0.01),40μmol/L组PPARγ、PRDM16的表达量为对照组的(3.88±0.31)、(1.22±0.13)倍(P<0.05)。结论 Res可能通过促进3T3-L1细胞线粒体的生物合成,减少其脂质蓄积,从而提升细胞能量代谢水平。
Objective To investigate the effect of resveratrol (Res) on the mitochondrial biosynthesis of 3T3-L1 adipocytes and to explore the role of resveratrol in cellular energy metabolism. Methods The 3T3-L1 preadipocytes were induced to differentiate. The control group and 10, 20 and 40μmol / L Res intervention groups were set up respectively. The lipid accumulation was observed by oil red O staining. The number and morphology of mitochondria were observed by transmission electron microscope. Western blot was used to detect mitochondrial synthesis of peroxisome proliferator-activated receptorγ (PPARγ), peroxisome proliferator-activated receptorγcoactivator -1α, PGC-1α), PR domain-containing 16 (PRDM16). Results Res intervention led to 10.7%, 38.9% and 62.9% reduction of lipid accumulation in 10, 20 and 40 μmol / L cells (P <0.01), respectively. The number of mitochondria increased, Increased; intracellular PPARγ, PGC-1α, PRDM16 protein expression increased. The expression of PPARγ and PGC-1α in 10μmol / L group was (1.91 ± 0.38) and (1.59 ± 0.10) times higher than that in control group (P <0.01) The expression levels of PPARγ and PRDM16 in 40μmol / L group were (3.88 ± 0.31) and (1.22 ± 0.15) times higher than that in control group (3.76 ± 0.28, 1.61 ± 0.15 and 1.30 ± 0.10) 0.13) times (P <0.05). Conclusion Res may promote the energy metabolism of 3T3-L1 cells by promoting the mitochondrial biosynthesis and decreasing its lipid accumulation.