Ticks are well known as vectors of many viruses which usually do great harm to human and animal health.Yunnan Province,widely covered by flourishing vegetation and mainly relying on farming husbandry,is abundant with Rhipi-cephalus microplus ticks.Therefore,it is of great significance to characterize the viral profile present in R.microplus parasitizing on cattle in Yunnan Province.In this study,a total of 7387 R.microplus ticks were collected from cattle and buffalo in the northwest and southeast areas of Yunnan Province from 2015 to 2017.We investigated the virome of R.microplus using next-generation sequencing (NGS) and the prevalence of important identified viruses among tick groups by RT-PCR.It revealed the presence of diverse virus concerning chu-,rhabdo-,phlebo-,flavi-and parvo-viruses in Yunnan.These viruses consist of single-stranded,circular and segmented sense RNAs,showing a greatly diversity in genomic organization.Furthermore,continuous epidemiological survey among ticks reveals broad prevalence of three viruses (Yunnan mivirus 1,Wuhan tick vrius 1 and YN tick-associated phlebovirus 1) and two possible prevalent viruses including a flavivirus-like segmented virus (Jingmen tick virus) and a bovine hokovirus 2 in Yunnan.Serological inves-tigation among cattle indicates that these identified viruses may be infectious to cattle and can elicit corresponding antibody.Our findings on R.microplus-associated viral community will contribute to the prevention of viral disease and tracking the viral evolution.Further analysis is needed to better elucidate the pathogenicity and natural circulation of these viruses.